NSG™ Variants Portfolio

NSG™
(005557)
NOD scid gamma

• Engrafts the widest range of solid and hematological cancers, including ALL and AML
• Most sensitive host for cancer stem cells when compared to NODscid or nude mice
• Longer lifespan than NOD scid; supports long-term engraftment studies and capabilities; >89 weeks median

• No thymic lymphomas, can be used for long & short-term experiments
• Sensitive to irradiation

N/A

Ishikawa et al., 2005Shultz et al., 2005

NSG-SGM3
(013062)
NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ

• Increased CD4+ FoxP3+ regulatory T cell population after CD34+ humanization
• Enhances human myelopoiesis and terminal differentiation after CD34+ humanization
• Increased efficiency of engrafting human acute myeloid leukemia (AML)
• Supports higher levels of human myeloid cell engraftment

• Compromised human stem cell regeneration
• Suppression of human erythropoiesis
• Reduction of human B-lymphopoiesis

• Immunology
• Oncology

Nicolini et al., 2004Wunderlich et al., 2010Billerbeck et al., 2011Miller, et al., 2013

NRG
(007799)
NOD Rag gamma

• Long-term multilineage hematopoeitic stem cell repopulation similar to NSG mice
• Engrafts human PBMC without irradiation similar to NSG
• Engrafts a wide range of solid and hematological cancers
• Greater tolerance to genotoxic agents compared to NSG

• No thymic lymphomas, can be used for long & short-term experiments
• Requires higher dose of irradiation to obtain human HSC engraftment
• The serum half-life of human IgG is greatly reduced in NSG-B2m due to defective FcRn function

• Immunology

Pearson et al., 2008Brehm et al., 2010Maykel et al., 2014

NSG-A2
(009617)
NOD.Cg-Mcph1^{Tg(HLA-A2.1)1Enge} Prkdc^scid Il2rg^tm1Wjl/SzJ

• High engraftment of HLA-A2-restricted immune cells after CD34+ humanization
• Antibody responses improved in a Dengue virus infection model
• Allow HLA restriction of developing human CD8 cells in humanized models, and provide improved platforms for viral infection and vaccine models

• No thymic lymphomas - can be used for long-term experiments
• Sensitive to irradiation

• Immunology
• HIV and Infectious Disease

Shultz et al., 2010 Strowig et al., 2009 Jaiswal et al., 2012

NSG-HLA-A2/HHD
(014570)
NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(HLA-A/H2-D/B2M)1Dvs/SzJ

• Enable functional CD4+ T cell responses to viral infection after CD34+ humanization
• Permit HLA restriction and enhance immune responses of human CD8 T cells
• Antibody responses improved in a Dengue virus infection model

• No thymic lymphomas - can be used for long-term experiments
• Sensitive to irradiation

• Immunology
• HIV and Infectious Disease

Shultz et al., 2010 Strowig et al., 2009 Jaiswal et al., 2012

NSG-DR1
(012479)
NOD.Cg-Tg(HLA-DRA*0101,HLA-DRB1*0101)1Dmz Prkdc^scid Il2rg^tm1Wjl/GckRolyJ

• Useful for transplantation studies in the absence of xeno-GVHD

• Reduced CD45+ cell engraftment compared to NSG

• Immunology
• HIV and Infectious Disease

Covassin et al., 2011

NSG-Ab^o DR4
(017637)
NOD.Cg-Prkdc^scid Il2rg^tm1Wjl H2-Ab1^b-tm1Doi Tg(HLA-DRB1)31Dmz/SzJ

• Useful for transplantation studies in the absence of xeno-GVHD
• Develop allo-GVHD post-engraftment of DR4-negative CD4+ T cells
• Show enhanced antibody responses, and human CD4+ T cells interact more effectively with human antigen presenting cells.

• Reduced CD45+ cell engraftment compared to NSG
• Higher proportion of mice with no CD45+ cell engraftment as compared to NSG

• Immunology
• HIV and Infectious Disease

Covassin et al., 2011Danner R et al., 2011

NSG B2m
(010636)
NOD.Cg-B2m^tm1Unc Prkdc^scid Il2rg^tm1Wjl/SzJ

• Resistant to xeno-GVHD
• Useful for studying mechanisms for xeno-GVHD

• N/A

• HIV and Infectious Disease
• Transplant Research

Covassin et al., 2013King et al., 2008

NSG-(K^bD^b)^null
(023848)
NOD.Cg-Prkdc^scid H2-K1^b-tm1Bpe H2-D1^b-tm1Bpe Il2rg^tm1Wjl/SzJ

• Attenuated xeno-GVHD development post-Hu-PBMC transplantation
• High Hu-CD45+ cell engraftment
• Useful for studying mechanisms for xeno-GVHD

• Reduced survival post Hu-CD34+ cell transplantation compared to NSG mice

• HIV and Infectious Disease
• Transplant Research

Covassin et al., 2013

NSG-MHC I/II DKO
(025216)
NOD.Cg-Prkdc^scid H2-K1^b-tm1Bpe H2-Ab1^g7-em1Mvw H2-D1^b-tm1Bpe Il2rg^tm1Wjl/SzJ

• Most resistant to xeno-GVHD of all NSG variants
• Extended human IgG half-life compared to B2m knockouts

• Resistance to xeno-GVHD associated with reduced CD45+ cell expansion in vivo
• Radiation sensitive

• Transplant Research

Brehm et al., 2019

NSG-Tg(Hu-IL15)
(030890)
NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(IL15)1Sz/SzJ

• Increased abundance and function of human NK cells following CD34-humanization

• Sensitive to irradiation

• Immunology

Brehm et al., 2019

NBSGW
(026622)
NOD.Cg-Kit^W-41J Tyr⁺Prkdc^scid Il2rg^tm1Wjl/ThomJ

• Human CD34 cells engraft effectively without preconditioning irradiation
• Longer lifespan than other immunodeficient Kit mutants
• Increased human erythroid cells (bone marrow)

• Although irradiation is not required for stem cell engraftment, NBSGW mice likely share the same sensitivity to genotoxic agents as other NSG strains

• Immunology

McIntosh et al., 2015

NSG-PiZ
(028842)
NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(SERPINA1*E342K)#Slcw/SzJ

• Engrafts human and allogeneic mouse hepatocytes without rejection

• Shares the same sensitivity to genotoxic agents as other NSG strains

• Transplant Research

Borel et al., 2017

NSG-TLR4 KO
(033704)
NOD.Cg-Tlr4^lps-del Prkdc^scid Il2rg^tm1Wjl/SzJ

• Residual mouse innate immune system cannot respond to Tlr4 agonists
• After humanization, NSG-Tlr4 KO are ideal for studying human TLR4-specific responses against tumors

• Shares the same sensitivity to genotoxic agents as other NSG strains

• Transplant Research

Aryee et al., 2019