A New Mouse Mutation on Chromosome 13 Causing a Striped Coat

A new mouse mutation on Chromosome 13 causing a striped coat

Authors:Belinda S. Harris, Patricia F.Ward-Bailey, Kenneth R. Johnson, Roderick T. Bronson

Source of Support: This research was supported by grants NIH/NCRR Grant RR01183 to the Mouse Mutant Resource (M.T. Davisson, PI) and Cancer Center Core Grant CA34196.

Mutation (allele) symbol: Mfs

Mutation (allele) name: Mutant fur is striped

Gene symbol:

Strain of origin:C3H/HeJ

Current strain name:C3H/HeJ-Mfs/J

Stock #:004806 (view JAX® Mice Data Sheet for additional information including Price and Supply Information)

Phenotype categories: skin and hair

Abstract

A dominant coat color mutation has been found in the Mouse Mutant Resource at the Jackson Laboratory and has been named Mutant fur is striped (Mfs). The mutants can be recognized by 3 weeks of age by their striped coats. The mutation maps to Chromosome 13.

Origin and Description

The Mfs mutation was found in a colony of C3H/HeJ mice bearing a different new mutation at generation F 226+F3. Mice affected by the Mfs mutation are recognized by a striped pattern in their coats that can be seen by three weeks of age. (See photo) The striping occurs in both males and females and in both homozygotes and heterzygotes. Both female and male mutants are fertile and have the expected ratio of mutants in their offspring. The C3H/HeJ-Mfs/J colony is currently maintained by wildtype x heterozygote matings.

Genetic Analysis

Using our standard mapping protocols a female affected by the Mfs mutation was mated to a CAST/Ei male mouse. The affected F1 progeny produced from this mating were then backcrossed to an unaffected mouse and produced 90 affected progeny of which 21 were used for linkage analysis. The Mfs mutation maps to Chromosome 13 between D13Mit16 (NCBI 36 position 20.3 Mb) and D13Mit10 (UCSC postion 49.7-49.9), and is non-recombinant with D13Mit60 (NCBI 36 position 35.9 Mb).

Pathology

A pathological screen of three mutants and one control mouse found no lesions. The eyes of two mutant mice and one control mouse were examined with an opthalamascope and all had retinal degeneration 1 ( Pde6b^rd1) which is normal for the C3H/HeJ background strain and not caused by the Mfs mutation. Hearing as assessed by auditory brainstem testing (ABR) of both mutant and control mice showed that all had normal hearing

Acknowledgements

The authors would like to thank Heping Yu for ABR testing, Norm Hawes for eye examinations, and Coleen Marden for pathological preparations.

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